Lactate Induces Cisplatin Resistance in S. cerevisiae through a Rad4p-Dependent Process

Figure 3

Effect of lactate on cisplatin-induced cell death of respiratory-deficient cells. Wild-type BY4741 and respiratory-deficient rho⁰ cells were grown to early exponential phase in SC-glucose medium (pH 5.5). Cells were washed and exposed to 600 μg/mL cisplatin in SC medium containing glucose or lactate for up to 180 minutes. Cell viability was assessed by semiquantitative spot assay on YPD plates. A representative image from 3 independent experiments is shown.