Research Article

Protective Effect of Astragaloside IV on High Glucose-Induced Endothelial Dysfunction via Inhibition of P2X7R Dependent P38 MAPK Signaling Pathway

Figure 8

Effect of P2X7R knockdown on P2X7R, p-P38 MAPK, inflammation, oxidative stress, eNOS, and NO in a high glucose environment. (a–c) Western blot analysis of protein levels of P2X7R, p-p38, and t-p38 MAPK with quantification (). (d–h) IL-6, TNF-α, IL-1β, and IL-18 proteins, and GSH-px activity were measured by ELISA (). (i) The level of SOD in RAOEC was measured using a SOD assay kit (). (j, k) The level of ROS was determined by DHE dye (). DHE dye (red). (l, m) Western blot analyses of the protein level of eNOS with quantification (). (n) The level of NO was tested using the Griess reaction (NO reaction end products: nitrate plus nitrite) (). vs. control group. #, ##, vs. HG group.