Functional Redox Proteomics Reveal That Salvia miltiorrhiza Aqueous Extract Alleviates Adriamycin-Induced Cardiomyopathy via Inhibiting ROS-Dependent Apoptosis

Figure 5

(a) Western blot analysis for phosphorylation and total protein levels of ERK1/2 and p53 with different treatments. The phosphorylation levels of ERK1/2 and p53 were normalized by total protein levels. Results represent the of three independent experiments (). GAPDH was applied as the loading control. (b) Cells were preincubated with or without U0126 for 0.5 h and then treated with or without ADR and SMAE for 24 h. The phosphorylation levels of ERK1/2 and p53 were normalized by total protein levels. β-Actin was used as an internal control. Results represent the of three independent experiments. (c) The protein levels of Bcl-xL and Bax with or without treatments of U0126, ADR, and SMAE were determined by western blotting assays. Density ratio of Bcl-xL over Bax was measured by densitometer, and β-actin was used as an internal control. The quantified results were indicated by the bar chart. (d) Cleavage of caspase-3 and PARP with or without treatments of U0126, ADR, and SMAE was determined by western blotting assays. β-actin was used as an internal control. The quantified results were indicated by the bar chart. (e) Cleavage of caspase-3 and PARP with or without treatments of Z-VAD-FMK (30 μM), ADR, and SMAE was determined by western blotting assays. β-Actin was used as an internal control. The quantified results were indicated by the bar chart. (f) SMAE efficacy on protection against ADR induced cathepsin B/AIF-mediated apoptosis. The quantified results were indicated by the bar chart. Results represent the of three independent experiments ().

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