Inhibition of JNK Alleviates Chronic Hypoperfusion-Related Ischemia Induces Oxidative Stress and Brain Degeneration via Nrf2/HO-1 and NF-κB Signaling

Figure 2

Chronic ischemia induced the stress kinase p-JNK activation and deregulates Nrf2/HO-1 proteins. (a, b) Western blot findings of p-JNK in the different samples of the experimental groups. (c, d) Showing ROS and LPO assay results. (e, f) Immunofluorescent images representing the immunoreactivity of p-JNK (FITC; green color, DAPI; blue color) both in the cortex and hippocampal regions. (g, h) Western blot results of Nrf2/HO1, in the cortex, and hippocampus of the experimental groups. (i, j) Immunofluorescence results of Nrf2/HO1 in the experimental groups. In confocal microscopy the Magnification were kept 10x, for all antibodies. In western blot assay, the β-actin was used as a loading control. Density values are relative to the control group (Rounding Rules) and expressed in arbitrary units (A.U). For western blot. The data are presented as the . Significantly different from the control group; ^#Significantly different from the ischemia group. , ^#P < 0.05.

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