Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells
Trehalose increased autophagosomes formation and autophagy flux. (a–e) Endogenous LC3II expression in cell lysate from ARPE-19 cells treated as designated. (f, g) Live-fluorescence microscopy showing percentage of GFP-LC3–expressing cells containing >10 GFP-LC3 puncta after treatment. (h) Free GFP levels in cell lysate from GFP-LC3–expressing cells after treatment. (i) Flow cytometry results of Annexin V/PI staining in wildtype cells after trehalose treatment. Percentage of late apoptosis and early apoptosis are shown in the upper right and lower right quadrants, respectively. Data represent the of 3 independent experiments. Statistical analysis was performed by one-way ANOVA followed by multiple comparison tests. ,,.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.