Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells

<div>Trehalose increased autophagosomes formation and autophagy flux. (a–e) Endogenous LC3II expression in cell lysate from ARPE-19 cells treated as designated. (f, g) Live-fluorescence microscopy showing percentage of GFP-LC3–expressing cells containing &gt;10 GFP-LC3 puncta after treatment. (h) Free GFP levels in cell lysate from GFP-LC3–expressing cells after treatment. (i) Flow cytometry results of Annexin V/PI staining in wildtype cells after trehalose treatment. Percentage of late apoptosis and early apoptosis are shown in the upper right and lower right quadrants, respectively. 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Oxidative Medicine and Cellular Longevity

fig1

Figure 1

Figure 1: Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells 

Figure 1 | Autophagy Upregulation by the TFEB Inducer Trehalose Protects against Oxidative Damage and Cell Death Associated with NRF2 Inhibition in Human RPE Cells 