Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 2

Research Article

O-GlcNAcylation Is Essential for Autophagy in Cardiomyocytes

Figure 2

Deletion of OGT in isolated neonatal cardiomyocyte attenuates autophagy. (a) Left panel, western blot analysis for autophagy of Ad-LacZ or Ad-Cre-infected Ogtf/f and/or Ogtf/y neonatal cardiomyocytes with either full medium (nutrient) or starving medium (starvation), using antibodies against OGT, O-GlcNAc, LC3, and SQSTM1. GAPDH was detected as a loading control. Right panel, quantification of western blot results in the left panel, for each group. Significantly different; M.W.: molecular weight. (b) Left panel, representative fluorescent images of adenovirus RFP-GFP-LC3-infected control (Ad-LacZ treated) and knockout (Ad-Cre treated) Ogtf/f and/or Ogtf/y neonatal cardiomyocytes. Quantitative analyses of LC3 puncta per cell were shown in the right panel. 50 cells were analyzed for each group. Significantly different.

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