Hypoxia-Induced Glioma-Derived Exosomal miRNA-199a-3p Promotes Ischemic Injury of Peritumoral Neurons by Inhibiting the mTOR Pathway

Figure 2

Effects of hypoxia on the proliferation of C6 and HT22 cells in the coculture system. (a) In the direct coculture system, the ratio of C6/HT22 cells was significantly higher under hypoxia after 3 days. vs. no hypoxia. (b) In the indirect coculture system, the proliferation ability of HT22 and C6 cells was similar under normal conditions, but when hypoxic conditions were applied, the MTT level of HT22 cells was lower than that of C6 cells in both the mono- and coculture systems. Moreover, HT22 cells had much lower MTT levels in the coculture system than in the monoculture system. vs. C6 glioma cells; ^# vs. HT22 cells monocultured under hypoxic conditions. ^& vs. HT22 cells monocultured under none hypoxic conditions. /group in at least 3 independent experiments.

(a)

(b)