Drp-1 as Potential Therapeutic Target for Lipopolysaccharide-Induced Vascular Hyperpermeability

Figure 3

Overexpression of Drp-1 upregulates LPS-induced mitophagy. PMVECs transfected with Drp-1 plasmid (a null-plasmid was used as the control) were exposed to LPS (500 ng/ml) and treated with 3MA (5 mM) or a vehicle for 24 h. (a) Western blotting assessment of TOMM20, TIMM23, PGC-1α, and TFAM levels; (b) TOMM20 level quantification; (c) TIMM23 level quantification; (d) TFAM level quantification; (e) PGC-1α level quantification. The represent the data ( in each group). An asterisk () indicates vs. the various groups. Drp-1: Dynamin-related protein-1; LPS: lipopolysaccharide; TOMM20: translocase of outer mitochondrial membrane 20; TIMM23: translocase of inner mitochondrial membrane 23; PGC-1α: PPARG coactivator 1 alpha; TFAM: transcription factor A mitochondria.

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