Research Article
Endoplasmic Reticulum Stress (ER Stress) and Unfolded Protein Response (UPR) Occur in a Rat Varicocele Testis Model
Table 1
List of primers used for real-time RT-PCR analyses.
| | Gene | GenBank accession nb. | Primer sequence (5-3)
F: forward; R: reverse | Amplicon size (bp) |
| | Grp78/BiP/HSPA5 | NM_013083.2 | F TAACAATCAAGGTCTACGAAGG
R CCATTCACATCTATCTCAAAGGT | 193 | | XBP1s | NM_001271731.1 | F CTGAGTCCGCAGCAGG
R CTTGTCCAGAATGCCCAAAAGG | 119 | | XBP1u | NM_001004210.2 | F GTCCGCAGCACTCAGACTAC
R CTGGGGAAGGACATTTGAAAAAC | 178 | | NRF2 | NM_031789.2 | F TGCCATTAGTCAGTCGCTC
R GTGCCTTCAGTGTGCTTC | 99 | | Bcl2 | NM_016993.1 | F ACTTCTCTCGTCGCTACCGTC
R AAGAGTTCCTCCACCACCGT | 106 | | Caspase-3 | NM_012922.2 | F CGGTATTGAGACAGACAGTGGAAC
R GCGGTAGAGTAAGCATACAGGAAG | 90 | | Bax | NM_017059.2 | F GGATCGAGCAGAGAGGATGG
R ACACTCGCTCAGCTTCTTGG | 91 | | Bak | NM_053812.1 | F CAGAGAGGTGGTTGGGTGG
R GTGGGTTGGGGAGAGGTTTAG | 181 | | Bim | NM_171988.2 | F CACAAACCCCAAGTCCTCC
R AGTCTCATTGAACTCGTCTCC | 152 | | GAPDH | NM_017008.4 | F TGCCGCCTGGAGAAACC
R TGAAGTCGCAGGAGACAACC | 121 |
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