Research Article

Hypoxia Helps Maintain Nucleus Pulposus Homeostasis by Balancing Autophagy and Apoptosis

Table 1

Sequences of rat primers used for real-time PCR.

Target geneSourceSequencePredicted length (bp)

GAPDHNM017008.4F: AAC TCC CTC AAG ATT GTC AGC AA
R: GGC TAA GCA GTT GGT GGT GC
51
Sox-9NM080403.1F: ACG GCT CCA GCA AGA ACA AG
R: TTG TGC AGA TGC GGG TAC TG
109
AggrecanJ03485.1F: GAC CAG GAG CAA TGT GAG GAG
R: CTC GCG GTC GGG AAA GT
72
Type I collagenNM053304.1F: TGG CCA AGA AGA CAT CCC TGA AGT
R: ACA TCA GGT TTC CAC GTC TCA CCA
81
Type II collagenNM012929.1F: GAG TGG AAG AGC GGA GAC TAC TG
R: CTC CAT GTT GCA GAA GAC TTT CA
81
Type III collagenNM032085.1F: TTC CTG GGA GAA ATG GCG AC
R: GGC CAC CAG TTG GAC ATG AT
99
Type VI collagenXM001079629.4F: CAA GAA CAC GTG GAC ATG CG
R: CAC TGC AGT TTC TTG ACG GC
77
ALPNM013059.1F: CAT GTT CCT GGG AGA TGG TA
R: GTG TTG TAC GTC TTG GAG AGA
144
Runx2NM001278483.1F: GAT GAC ACT GCC ACC TCT GA
R: ATG AAA TGC TTG GGA ACT GC
118
BMP-2NM017178.1F: CTA TAT GCT CGA CCT GTA CCG
R: CAC TCA TTT CTG AAA GTT CCT CG
146
TGF-βNM_021578.2F: CGC AAC AAC GCA ATC TAT G
R: ACC AAG GTA ACG CCA GGA
204
TIMP-1NM053819.1F: TCC CCA GAA ATC ATC GAG AC
R: TCA GAT TAT GCC AGG GAA CC
250
TIMP-2NM021989.2F: CAG GGC CAA AGC AGT GAG CGA GAA
R: TCT TGC CAT CTC CTT CCG CCT TCC
230
MMP-2NM031054.2F: GAT CTG CAA GCA AGA CAT TGT CTT
R: GCC AAA TAA ACC GAT CCT TGA A
83
MMP-3NM133523.2F: TCC CAG GAA AAT AGC TGA GAA CTT
R: GAA ACC CAA ATG CTT CAA AGA CA
74
MMP-9NM031055.1F: GTA ACC CTG GTC ACC GGA CTT
R: ATA CGT TCC CGG CTG ATC AG
68
MMP-13NM133530.1F: CTG ACC TGG GAT TTC CAA AA
R: ACA CGT GGT TCC CTG AGA AG
96
HIF-1XM006240199.3F: AAG TCT AGG GAT GCA GCA C
R: CAA GAT CAC CAG CAT CTA G
175
SIRT1XM017588054.1F: AGC TGG GGT TTC TGT TTC CTG TGG
R: TCG AAC ATG GCT TGA GGA TCT GGG A
111
HMGB-1NM012963.2F: CGG ATG CTT CTG TCA ACT TCT
R: AGT TTC TTC GCA ACA TCA CCA
292
Beclin-1NM001034117.1F: TTC AAG ATC CTG GAC CGA GTG AC
R: AGA CAC CAT CCT GGC GAG TTT C
142
Atg7NM001012097.1F: GAC CTG GGC TCC TCA CTT TTT G
R: CCC TGG GCG GCT CAC TG
135
Atg5NM001014250.1F: AGG CTC AGT GGA GGC AAC AG
R: CCC TAT CTC CCATGG AAT CTT CT
72
LC3NM022867.2F: CAT GCC GTC CGA GAA GAC CT
R: GAT GAG CCG GAC ATC TTC CAC T
70
LC3-IINM022867.2F: CTT TGT AAG GGC GGT TCT
R: GAG GCT TGC TTT AGT TGG
141
p53NM030989.3F: CAG CTT TGA GGT TCG TGT TTG T
R: ATG CTC TTC TTT TTT GCG GAA A
82
p21NM080782.3F: CAG ACC AGC CTA ACA GAT TTC
R: TGA CCC ACA GCA GAA GAA G
105
BaxNM017059.2F: CCA AGA AGC TGA GCG AGT GTC TC
R: AGT TGC CAT CAG CAA ACA TGT CA
147
Bcl-2NM016993.1F: TGA ACC GGC ATC TGC ACA C
R: CGT CTT CAG AGA CAG CCA GGA G
116
Caspase-3NM012922.2F: CTG GAC TGC GGT ATT GAG AC
R: CCG GGT GCG GTA GAG TAA GC
104
Caspase-8NM022277.1F: TCA GCA ACA TGC GGG ACA G
R: TGA AGC AGT CTT TGC CCT TGT G
171
Caspase-9NM031632.1F: GGA AGA TCG AGA GAC ATG CAG
R: CCG TGA CCA TTT TCT TAG CAG
216