A Dihydroflavonoid Naringin Extends the Lifespan of C. elegans and Delays the Progression of Aging-Related Diseases in PD/AD Models via DAF-16

Figure 2

Naringin delayed aging-related decline of phenotypes and improved stress resistance. (a) Aging-related movements of worms with nontreated control plates and 50 μM naringin. The mean body movement speed is found in Table S2 (Supplementary information). (b) The intestinal autofluorescence of lipofuscin was analyzed on the 2nd and 5th days of adulthood. The figures showed the mean lipofuscin aggregated in the intestinal tract at least repeated twice. The statistical details and repeats of these experiments are summarized in Table S3 (Supplementary information). (c) The survival curves of wild-type worms cultured at 20°C in plates with treated 50 μM and nontreated naringin on the 7th day of adulthood; then, the worms were exposed to paraquat (20 mM) and cultured at 20°C; and their death was calculated every day. Statistical details and repeats of these experiments are summarized in Table S4 (Supplementary information). (d) The survival curves of wild-type worms cultured at 35°C in nontreated control plates and plates treated with 50 μM naringin. Statistical details and repeats of these experiments are summarized in Table S5 (Supplementary information). (e) Survival curves of wild-type (N2) animals feeding Pseudomonas aeruginosa raised at 20°C on NGM plates in the absence (0 μM) or presence (50 μM) of naringin. Statistical details and repeats of these experiments are summarized in Table S1 (Supplementary information). (f) Survival curves of hsf-1 mutants in the control or treated with 50 μM naringin; naringin could not further extend the mean lifespan of C. elegans. Statistical details and repeats of these experiments were summarized in Table S1 (Supplementary information).

(a)

(b)

(c)

(d)

(e)

(f)