A Dihydroflavonoid Naringin Extends the Lifespan of C. elegans and Delays the Progression of Aging-Related Diseases in PD/AD Models via DAF-16

Figure 5

Naringin could affect the mRNA expression and not extend the lifespan of daf-2, akt-1, and akt-2 mutants in the IIS pathway. (a) The mRNA expression level of genes associated with DAF-16 and itself. The columns showed the mean value of two independent experiments with error bars representing SEM. represents , calculated using two-tailed -test. Statistical details and repeats of these experiments are summarized in Table S9 (Supplementary information). (b–d) Survival curves of daf-2, akt-1, and akt-2 mutants in the control or treated with 50 μM naringin; naringin could not further extend the mean lifespan or control plates without naringin. Statistical details of mutants and repeats of these experiments are summarized in Table S1 (Supplementary information).

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