Olive Leaf Polyphenols Attenuate the Clinical Course of Experimental Autoimmune Encephalomyelitis and Provide Neuroprotection by Reducing Oxidative Stress, Regulating Microglia and SIRT1, and Preserving Myelin Integrity

Figure 8

(a) Representative immunofluorescent pictures show the relationship between iNOS⁺ and Iba-1⁺ (M1) microglia cells in the subventricular zone: untreated, with induced EAE and the second attack (on the 20^th day postinduction), with induced EAE and treated with TOL till the 20^th day postinduction, with induced EAE and the second remission (on the 30^th day postinduction), and with induced EAE and treated with TOL on the 30^th day postinduction. (b) The number of Iba-1⁺ iNOS⁺ was manually counted in the area of interest (). Values are expressed as of a number of cells per mm². One-way ANOVA followed by the post hoc Scheffé test: . Scale bars indicate 50 μm and 20 μm (insets).

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