Research Article

Ameliorating Effect of Klotho Protein on Rat Heart during I/R Injury

Figure 5

Oxidative status in the hearts subjected to I/R injury: (a) total ROS and RNS level expressed as nM of DCF and normalized to coronary flow; (b) total antioxidant capacity of hearts subjected to I/R. TAC was expressed as μM of CRE and normalized to total protein concentration; (c) correlation of ROS/RNS level and LDH activity. CF: coronary flow; CRE: copper reducing equivalents; DCF: 2, 7-dichlorodihydrofluorescein; LDH: lactate dehydrogenase; mU/mL: milli international enzyme units per millilitre; RNS: reactive nitrogen species; ROS: reactive oxygen species; TAC: total antioxidant capacity; ; ; ; ; vs. Aero; # vs. I/R; ANOVA.
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