Allicin Attenuated Advanced Oxidation Protein Product-Induced Oxidative Stress and Mitochondrial Apoptosis in Human Nucleus Pulposus Cells

Figure 1

AOPP treatment inhibited human NP cell viability and proliferation in a dose- and time-dependent manner. (a) The human NP cells were treated with AOPP (0, 100, 200, and 400 μg/ml) for 24 h, and 0 μg/ml group served as a control. The cell viability of each group was detected by a CCK-8 assay. (b) The human NP cells were treated with AOPP (0-400 μg/ml) for 24 h, and 0 μg/ml group served as a control. The cell proliferation was determined using EdU staining combined with DAPI staining for the nuclei under fluorescence microscope, with the EdU positive cells quantitated. Original magnification: ×200. (c) The human NP cells were treated with 400 μg/ml AOPP for 0 h, 2 h, 6 h, 12 h, and 24 h, and 0 h group served as a control. The cell viability of each group was detected by a CCK-8 assay. (d) The human NP cells were treated with 400 μg/ml AOPP for 0 h, 2 h, 6 h, 12 h, and 24 h, and 0 h group served as a control. The cell proliferation was determined using EdU staining combined with DAPI staining for the nuclei under fluorescence microscope, with the EdU positive cells quantitated. Original magnification: ×200. Data were represented as . and versus the control group, .

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