Sustained Release of Melatonin from GelMA Liposomes Reduced Osteoblast Apoptosis and Improved Implant Osseointegration in Osteoporosis

Figure 4

GelMA-DOPA@MT inhibits the apoptosis of osteoblasts in vitro. (a) Representative images showing TUNEL staining, with yellow arrows indicating apoptotic cells. Scale bar: 50 μm. (b) Quantitative analysis of TUNEL staining results. per group, , , vs. the GelMA-DOPA@MT group. (c) Cell lysate was subjected to Western blotting with antibodies against the apoptosis-specific proteins Bax and Bcl-2. (d–f) Quantification of Bax and Bcl-2 protein levels, per group, , , vs. the GelMA-DOPA@MT group. (g–i) qPCR analysis of the expression of the apoptosis-specific genes Bcl-2 and Bax in the experimental group. per group, , , vs. the GelMA-DOPA@MT group.

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