A Novel Molecular Mechanism of IKKε-Mediated Akt/mTOR Inhibition in the Cardiomyocyte Autophagy after Myocardial Infarction
IKKε is essential for H9c2 cell survival under starvation injury. H9c2 cells were infected with siRNA-control and LV-control, siRNA-IKKε and Over-control, and siRNA-IKKε and Over-IKKε three groups with or without starvation injury, a total of 6 groups. (a, b) Representative western blots and quantitative results of IKKε, LC3I/II, ATG-5, C-caspase 3, Bcl-2, and Bax in H9c2 cells of each 6 groups. ;;. (c) Representative immunofluorescent staining of LC3 in H9c2 cells of each 6 groups ( for each group; magnification, 600x). Scale bar represents 10 μm. (d) The quantification of autophagy is expressed as the number of LC3 puncta per area (for each group). .
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