Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway
GSC extracts prevent HG/PA-induced cellular senescence in HAECs. HAECs were treated with varying concentrations of GSC extracts in the presence of 40 mM glucose and 0.1 mM palmitate for 48 h. (a) Cell viability as determined by CCK-8 assay. (b) SA-β-gal were detected in HAECs. (c) Western blot analysis of the expression of p16 and p21 in HAECs. (d) Confocal images to show γ-H2AX focus expression in HAECs after 48 h. # and ##, compared with the control group; and , compared with the model (HG/PA) group.