Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway
Mdivi-1 pretreatment for 1 h abolished the induction of mitophagy by GSC extracts. (a, b) Western blot analysis of the expression of PINK1 and Parkin protein in cultured HAECs after pretreatment with 2 μM Mdivi-1. (c) Image shows the colocalization of Parkin protein and mitochondria in HAECs after pretreatment with 2 μM Mdivi-1. (d) HAECs were treated with Mdivi-1 for 48 h followed by treatment with 20 μM CCCP for 4 h. Image shows the colocalization of GFP-LC3 with mitochondria after treatment with CCCP. (e) Pearson correlation coefficient shows the degree of colocalization between LC3 and mitochondria. (f, g) Western blot analysis of LC3B-II and p62 for 48 h in cultured HAECs after pretreatment with 2 μM Mdivi-1. # and ##, compared with the control group; and , compared with the model (HG/PA) group.