Notoginseng Leaf Triterpenes Ameliorates OGD/R-Induced Neuronal Injury via SIRT1/2/3-Foxo3a-MnSOD/PGC-1α Signaling Pathways Mediated by the NAMPT-NAD Pathway

Figure 3

Effects of PNGL on the MMP, ROS levels, and the Nampt-SIRT1/2/3 pathway in OGD-induced SH-SY5Y cells. PNGL raised MMP, reduced the ROS levels, and upregulated the Nampt and SIRT1/2/3 expression in OGD/R-induced SH-SY5Y cells, partly reversed by the inhibitor FK866. (a) The ROS fluorescence images of PNGL in OGD/R-induced SH-SY5Y cells, measured by a fluorescence microscope. (b) The JC-1 fluorescence images of PNGL on MMP, measured with the JC-1 assay by a fluorescence microscope. The statistical data of fluorescence value, analyzed using the Image J 2.44 software; scale bar, 200 μm. (c) The protein bands were examined by western blot analysis. (d–g) The relative expression levels were quantified and analyzed by using Gel-Pro analyzer software. The data presented as the . ^#, ^## versus the control group; , versus the OGD/R model group; ^&, ^&& versus the OGD/R+PNGL group.

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