Characterization of conditionally reprogrammed lung cancer cells (CRLCs). (a) The morphology of CRLCs in different growth periods. (b–d) Representative immunofluorescence images of CRLCs, 3T3-J2, and a lung adenocarcinoma cell line SPCA-1 showing phenotypic classifiers for CK7, CEA, and TTF-1. The nuclei were counterstained with Hoechst 33342 for DNA. (e) Immunocytochemistry staining of CRLCs, 3T3-J2, and SPCA-1 with the indicated markers CK7, CEA, and TTF-1. (f) Western blot analysis of CRLCs, 3T3-J2, and SPCA-1 with the following markers: CK7, CEA, and TTF-1. (g) STR analysis of CRLCs. All Western blot band intensities were normalized to GAPDH.