Pristimerin Exacerbates Cellular Injury in Conditionally Reprogrammed Patient-Derived Lung Adenocarcinoma Cells by Aggravating Mitochondrial Impairment and Endoplasmic Reticulum Stress through EphB4/CDC42/N-WASP Signaling

Figure 2

Effect of PRIS on cell proliferation and colony formation ability in CRLCs. (a) Cells were treated with 0 (control), 1, 2, 4, 8, and 16 μM PRIS for 24, 48, and 72 h followed by MTS assay to determine cell number/cell proliferation, with cells that received no treatment as the negative control (). (b) Cells were treated with the same concentrations as in (a) for 24 h, and cell death was evaluated by LDH assay (). (c) Cell survival was monitored using a calcein-AM/EthD-1 double staining. Micrographs representing cells stained with calcein-AM and EthD-1 after treating the cells for indicated doses for 24 h. Viable cell population appears green (calcein-AM) whereas nonviable/dead cells appear as red (EthD-1) in the fluorescent micrographs. (d) CRLCs were treated with PRIS (0, 1, 2, 4, 8, and 16 μM) for 24 h and then, after withdrawal of the treatment, were left to grow for 3 weeks. A colony formation assay was performed to evaluate the survival of colony-forming cells (number of colonies) (). Data are presented as , .

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