Research Article
[Retracted] An Immunohistochemical Study of the Increase in Antioxidant Capacity of Corneal Epithelial Cells by Molecular Hydrogen, Leading to the Suppression of Alkali-Induced Oxidative Stress
Figure 2
Macroscopical images of eyes and increased ROS production and antioxidant enzyme expression after H2 pretreatment. The animals were sacrificed after three-day pretreatment of eyes with H2 or buffer and the corneas examined. The production of ROS, SOD, and CAT expressions in the corneal epithelium of eyes pretreated with H2, is higher compared to that in the control corneal epithelium or corneal epithelium pretreated with buffer. The intensities of DHE, SOD, and CAT expressions are quantified. The values with asterisks are significantly different (, ) from buffer-pretreated corneas. Buffer-pretreated DHE ; H2-pretreated DHE ; control DHE ; buffer-pretreated SOD ; H2-pretreated SOD ; control SOD ; buffer-pretreated CAT ; H2-pretreated CAT ; control CAT ; /group. Scale bars: 50 μm.