Oleanolic Acid Decreases IL-1β-Induced Activation of Fibroblast-Like Synoviocytes via the SIRT3-NF-κB Axis in Osteoarthritis

Figure 1

The effect of OLA on human FLS and the expression of SIRT3. (a) Human FLS were treated with increasing concentrations of OLA (0, 1, 10, 20, 50, and 100 μM) for diffident time periods (24 h, 28 h, and 72 h). The effects of OLA on human FLS viability were evaluated via the CCK-8 assay. (b) After treatment with different concentrations of OLA for 24 h, the SIRT3 expression in human FLS was detected using RT-PCR. (c) An immunohistochemical stain was performed to evaluate the expression of SIRT3 in the OA synovium. (d) The mRNA expression of SIRT3 from donors between normal synovial tissue and from the OA synovium. Normal group (4 females, mean age 45 yrs); OA synovium group (4 females, mean age 63 yrs). (e) The mRNA expression of SIRT3 decreased by IL-1β in FLS. FLS were treated with 10 ng/ml of IL-1β for different time periods (0, 1, 6, 12, 24, and 48 h). The expression of SIRT3 significantly decreased at 6, 12, 24, and 48 h compared with 0 h. The values are expressed as (SD), . .

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