Sirtuin 3 Inhibits Airway Epithelial Mitochondrial Oxidative Stress in Cigarette Smoke-Induced COPD
Sirt3 regulated the expression and activity of MnSOD in airway epithelial cells exposed to CSE. Real-time PCR showed that the declines in Sirt3 (a) and MnSOD (b) expressions were significantly intensified by Sirt3 siRNA and improved by Sirt3 overexpression in CSE-treated BEAS-2B cells. A representative blot of Sirt3 and MnSOD expressions determined by Western blot is shown for each condition (c). Further quantification analysis revealed that the deceases in protein expression of Sirt3 (d) and MnSOD (e) were significantly aggravated by Sirt3 siRNA and reversed by Sirt3 overexpression when BEAS-2B cells were stimulated with 7.5% CSE. The decline in MnSOD activity was significantly intensified by Sirt3 siRNA and prevented by Sirt3 overexpression in BEAS-2B cells exposed to 7.5% CSE (f). All values are shown as from three independent experiments. versus control group, and # versus 7.5% CSE-treated group.