Piquiá Shells (Caryocar villosum): A Fruit by-Product with Antioxidant and Antiaging Properties in Caenorhabditis elegans

Figure 5

Effect of Caryocar villosum fruit shells (CV) on superoxide dismutase 3 (sod-3) expression under basal and mild oxidative stress, induced by a dose of 20 μM juglone. (a) sod-3::GFP expression levels under basal stress conditions. (b) sod-3::GFP expression levels under juglone-induced oxidative stress. (c) Relative sod-3 mRNA levels of wild-type worms under basal stress conditions. (d) Relative sod-3 mRNA levels of wild-type worms under 20 μM juglone-induced oxidative stress. Data are representative of three independent experiments. GFP expression levels are expressed as (, , and , compared to the respective control) (^##, compared to the untreated control). Relative mRNA levels are presented as mean fold relative mRNA levels, calculated from ΔΔCt values normalized to cdc-42 as a reference gene (, , compared to juglone).

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