Research Article

Downregulated Recycling Process but Not De Novo Synthesis of Glutathione Limits Antioxidant Capacity of Erythrocytes in Hypoxia

Figure 4

Hypoxia downregulates the glucose metabolism of RBCs exposed to oxidative stress. (a and b) ECAR in normoxic and hypoxic RBCs after HX/XO exposure (1.5 mM HX; 0.4 U/ml XO; 30 min). (c) The activity of GAPDH, the rate-limiting enzyme of EMP, in normoxic and hypoxic RBCs. (d) The activity of G6PD, the rate-limiting enzyme of HMP, in normoxic and hypoxic RBCs. The levels of ATP (e) and NADPH (f) in normoxic and hypoxic RBCs. Data represent the of at least three independent experiments. and for normoxic or hypoxic RBCs vs. corresponding RBCs with HX/XO exposure. # for normoxic RBCs vs. corresponding hypoxic RBCs.
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