FoxC1-Induced Vascular Niche Improves Survival and Myocardial Repair of Mesenchymal Stem Cells in Infarcted Hearts
FoxC1-induced vascular niche at 15 days after MI. MI rat hearts were transfected with Foxc1 vector (adFoxc1), FoxC1 siRNAs (siFoxC1), or control siRNA duplexes (CON) immediately after MI induction. (a) Real-time qRT-PCR quantitative analysis data of FoxC1, Ang-1, bFGF, and VEGF mRNA in the adFoxC1 group, CON group, and siFoxC1 group 15 d post-MI. (b) Immunoblotting analyses show the representative expression of FoxC1 and its downstream growth factors. (c) Quantification data of vessel numbers in these groups. (d) Immunohistochemical staining showing positive staining (brown) of FoxC1, Ang-1, bFGF, and VEGF at the peri-infarct area. Representative slides show factor VIII+ ECs in the peri-infarct regions from the adFoxC1 group, CON group, and siFoxC1 group. . All data are the .: vs. adFoxC1 group, †vs. CON group ( per group); Student’s -test.
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