Tribulus terrestris Ameliorates Oxidative Stress-Induced ARPE-19 Cell Injury through the PI3K/Akt-Nrf2 Signaling Pathway

Figure 2

EE-TT inhibited H₂O₂-induced apoptosis in ARPE-19 cells. (a) ARPE-19 cells with or without H₂O₂ treatment for 24 h, then cells were treated with or without ethanol extracts of Tribulus terrestris (EE-TT) (100 or 200 μg/mL) for another 24 h, the cells were stained with annexin V-FITC/PI double staining and analyzed by flow cytometry. The percentage of cells in each quadrant is presented. (b) The quantification of annexin V-FITC-positive cells () was calculated for each group cells and is shown in the bar graph. (c) The quantification of early apoptotic cells (Q4) was calculated for each group cells and is shown in the bar graph. The data in (b) and (c) are shown as , , , vs. the samples of control group (nontreated cells); ^#, ^##, ^### vs. the samples treated with H₂O₂ alone.

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