Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 3

Research Article

Tribulus terrestris Ameliorates Oxidative Stress-Induced ARPE-19 Cell Injury through the PI3K/Akt-Nrf2 Signaling Pathway

Figure 3

Effects of EE-TT on the protein expression levels of Bcl-2, Bax, caspase-3, and caspase-9 in H2O2-treated ARPE-19 cells. ARPE-19 cells were pretreated with or without 1 mM H2O2 for 24 h, followed by a 24 h exposure to ethanol extracts of Tribulus terrestris (EE-TT) (100 or 200 μg/mL). (a) The cell lysates of control (nontreatment), H2O2-treated alone, or H2O2 plus EE-TT-treated samples were analyzed by Western blot assay with the antibodies of Bcl-2, Bax, cleaved caspase-3, cleaved caspase-9, and β-actin, respectively. Quantification data of these protein expression levels were measured and normalized to β-actin expression levels. The fold changes vs. control sample (nontreatment) were presented in the bar graph from (b–e), respectively. Data are shown as , , , vs. the nontreatment control sample; #, ##, ### vs. the sample with H2O2 treatment alone, .

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