Gender Difference on the Effect of Omega-3 Polyunsaturated Fatty Acids on Acetaminophen-Induced Acute Liver Failure
Estrogen is responsible for the sex-related function of n-3 PUFAs on APAP hepatotoxicity. (a–c) 100 mg/kg E2 was intraperitoneally injected into male WT or fat-1 mice 7 days before 400 mg/kg APAP administration (). (a) 24 hours after APAP injection, histological analysis of livers was performed by H&E staining. . (b, c) Serum ALT and LDH levels in various time points after APAP administration was evaluated. (d) HepaRG cells were pretreated with 50 μM DHA with or without 100 nM E2, followed by stimulation with 20 mM APAP for 24 hours. The cells were harvested and stained with Annexin V-FITC and PI for FACS analysis. . The data represent three independent experiments with similar results.