Overexpression of LMP-1 Decreases Apoptosis in Human Nucleus Pulposus Cells via Suppressing the NF-κB Signaling Pathway

Figure 2

The construction of LMP-1 overexpression and lenti-control NPCs. (a) NPCs after lentiviral transfection and puromycin screening were observed under a normal microscope and a fluorescence microscope. (b) Cell viability was measured by CCK-8 on days 1 and 3. (c) The gene expression level of LMP-1 was determined by RT-qPCR and normalized to 18 s. (d) The protein expression of LMP-1 in each group was determined by western blotting analysis. (e) The protein expression of LMP-1 was quantified. Data represent ; , vs. the mock treated group. .

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