Overexpression of LMP-1 Decreases Apoptosis in Human Nucleus Pulposus Cells via Suppressing the NF-κB Signaling Pathway

Figure 5

LMP-1 overexpression inhibited the activation of NF-κB signaling pathway. (a) Protein expression levels of pho-p65, p65, pho-IκBα, and IκBα of NPCs in each group were measured by western blotting analysis on day 3. (b) The protein expression was quantified according to signal intensity, and the ratio of pho-p65/p65 and pho-IκBα/IκBα in each group was calculated. siRNA for LMP-1 was transfected into NPCs, and the protein expression levels of pho-p65, p65, pho-IκBα, and IκBα of NPCs in each group were (c) measured and (d) quantified on day 3. Data represent ; , .

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