Differential Gender-Dependent Patterns of Cardiac Fibrosis and Fibroblast Phenotypes in Aging Mice

Figure 4

Differential gender-dependent distribution of fibroblast populations in the young and aged hearts. (a) Perivascular coronary, epicardial and myocardial immunofluorescence staining (488 nm) for PDGFRα of histological sections obtained from young and aged mice of both sexes, as well as quantifications of the respective stained zones. Dark blue arrows indicate cardiomyocytes nuclei stained with DAPI, whereas light blue arrows indicate co-localization of DAPI and PDGFRα in cardiac fibroblasts. White asterisks indicate coronary vessels. (b) Immunofluorescence staining (594 nm) for α-SMA of histological sections obtained from young and aged mice hearts of both sexes, as well as quantifications of the respective stained zones. Nuclei are stained blue with DAPI. Scale bars: 50 μm. Magnifications: x200. n =8 animals per group, fields of view per condition. , and vs young male and female, vs aged female or male.

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