Exosomal CircHIPK3 Released from Hypoxia-Induced Cardiomyocytes Regulates Cardiac Angiogenesis after Myocardial Infarction
HPC-exos promote cardiac endothelial cell migration, proliferation, and tube formation. Cardiac endothelial cells were incubated with depleted exos, Nor-exos, or HPC-exos for 24 h after exposure to 200 μM H2O2 for 3 h. (a) Cell proliferation was detected by using the EdU assay. (b) Quantification of the data presented in (a). (c) The migration ability of cardiac endothelial cells was measured by Transwell assay (original magnification, ×200). (d) Quantification of the data presented in (c). (e) Tube formation in Matrigel was assessed. (f, g) Quantitative assessment of the number of branch points and tubes formed. (h) The protein expression levels of cyclinD1 and PCNA were determined using Western blot analysis. (i) Quantification of the relative expression levels of cyclinD1 and PCNA. The results are normalized to the control (,;, compared with the normal group; # compared with the H2O2 group; and & compared with the Nor-exo group).
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