Chaperone-Mediated Autophagy Markers LAMP2A and HSC70 Are Independent Adverse Prognostic Markers in Primary Resected Squamous Cell Carcinomas of the Lung

Figure 1

CMA pathway and validation of LAMP2A immunohistochemical staining. (a) Overview of chaperone-mediated autophagy (CMA). ① Recognition and binding of HSC70 to the KFERQ-motif of the target protein. ② Translocation of the complex to the lysosome. ③ Binding of the target protein to LAMP2A at the lysosomal membrane. ④ Formation of a multimeric LAMP2A complex. ⑤ Translocation and degradation of the target protein. (b, c) Specificity of LAMP2A immunohistochemistry. SKBR3 cells were transduced with lentiviral vectors containing a LAMP2A cDNA (OE) construct, an empty vector control, or shRNAs targeting LAMP2A mRNA (shLAMP2A_1-2). After selection, cells were subjected to LAMP2A western blot analysis (b) and immunohistochemistry (c).

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