HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-κB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function

Figure 4

HSP60 affects the activation of the NLRP3 inflammasome in MSU crystal-treated THP-1-derived macrophages. (a–c) THP-1-derived macrophages were transfected with control siRNA or HSP60 siRNA for 48 h, primed with LPS (100 ng/ml) for 1 h, and then treated with MSU suspension (50 μg/ml) for 12 h. (a) Effect of HSP60 knockdown on NLRP3 mRNA levels. (b) Influence effect of HSP60 knockdown on NLRP3 protein levels. (c) Effect of HSP60 knockdown on the expression of the p20 subunit of Caspase-1 and IL-1β in the cell lysates and culture supernatants. (d) THP-1-derived macrophages were transfected with a control vector or human HSP60 vector for 36 h, primed with LPS (100 ng/ml) for 1 h, and then treated with MSU suspension (50 μg/ml) for 12 h. Effect of HSP60 overexpression on the expression of the p20 subunit of Caspase-1 and IL-1β in the cell lysates and culture supernatants. The p20 subunit of Caspase-1 protein levels in the cell lysates was normalized to ɑ-Tubulin and then averaged. Values are the of 3 independent experiments. .

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