HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-κB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function

Figure 6

HSP60 downregulation relieved the severity of MSU crystal-induced inflammatory mouse model. (a) Mice injected with HSP60-MO presented decreased the paw swelling index ( per group, ). (b) Immunostaining was used to detect HSP60 expression in the foot pad tissue section ( per group, ). Blue shows nuclei staining with DAPI. Scale bar: 20 μm. (c) HSP60 protein levels were analyzed by western blot in the foot pad tissue ( per group, ). (d) HE staining was used to observe the infiltration of inflammatory cells in the foot pad tissue sections ( per group, ). Images were obtained using a light microscope (Olympus, Tokyo, Japan) with 10 times or 20 times objectives and analyzed with image processing software (Olympus). (e) Immunostaining using anti-CD11b, anti-Ly-6G, and anti-MPO antibody in the foot pad tissue sections ( per group, ). Blue shows nuclei staining with DAPI. Scale bar: 40 μm. (f) Protein levels of MPO, iNOS, and COX-2 in the foot pad tissue were measured by western blot ( per group, ). (g) The MPO activity of foot pad tissue ( per group, ). ^#Significantly different from the absence of MSU crystals injection mice and significantly different from MSU crystals and ctrl MO injection mice.

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