The Inhibition of miR-873 Provides Therapeutic Benefit in a Lipopolysaccharide-Induced Neuroinflammatory Model of Parkinson’s Disease

Figure 1

The effects of the miR-873 inhibitor on the damage to DA neurons in the substantia nigra pars compacta in a LPS-induced rat model of PD. The animals were transfected with the miR-873 sponge 3 days before LPS treatment or 8 and 16 days after LPS treatment (a). The damage to DA neurons following LPS treatment was detected by immunohistochemistry staining () (b). The reduction in the tyrosine hydroxylase- (TH-) positive cells on the lesioned side was attenuated in the rats transfected with the miR-873 sponge 3 days before LPS treatment or 8 days after LPS injection, compared with LPS treatment alone (c). The accumulation of α-synuclein in DA neurons was examined by fluorescence immunohistochemistry () (d and e). The number of apomorphine-induced rotations following LPS treatment was decreased in the rats treated with the miR-873 sponge compared with the rats treated with LPS alone () (f). The mRNA levels of miR-873 were increased by LPS treatment, compared with the control () (g). Transfection of the miR-873 sponge attenuated the inhibition of the mRNA levels of ABCA1 (h) and A20 (i) following LPS treatment. The data are expressed as the ; , , and compared with the controls.

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