Polydatin Attenuates 14.1 MeV Neutron-Induced Injuries via Regulating the Apoptosis and Antioxidative Pathways and Improving the Hematopoiesis of Mice

Figure 8

Immunohistochemistry analysis of HO-1, KEAP 1, and SIRT 1 using mouse spleen from different groups. The spleens from at least 4 mice each group were isolated and fixed in 4% formaldehyde and embedded in paraffin. Sections were stained with DAB and incubated with the corresponding antibodies. A whole slide scanning method was utilized to obtain the digital pictures which were then applied to calculate the IHC staining intensity score via the IHC profiler plugin in the ImageJ software. (a) Representative IHC images of each group; (b) quantified percentage score of IHC pictures from at least eight high-power fields (×400) every group was subjected to statistical analysis using a two-tailed unpaired -test. The experiments were performed in triplicates, and data are presented here as . , .

(a)

(b)