Resveratrol Mitigates Sevoflurane-Induced Neurotoxicity by the SIRT1-Dependent Regulation of BDNF Expression in Developing Mice

Figure 1

Experiment protocol in vivo and in vitro. (a) In vivo: 3-day-old mice were pretreated with DMSO or resveratrol via intraperitoneal injection daily for 6 consecutive days and received control gas exposure or 3% sevoflurane for 2 h from P6 to P8. After exposure, some of the mice were decapitated and tissue samples were collected for RT-PCR and western blotting; the other mice were returned to dams and received the OF test and MWM test from P30 to P35. (b) In vitro: after 5-day culture, primary neurons were incubated with DMSO or 20 μM resveratrol followed by 6-hour exposure to control gas or 4% sevoflurane.

(a)

(b)