Research Article

Role of Mesencephalic Astrocyte-Derived Neurotrophic Factor in Alcohol-Induced Liver Injury

Figure 4

Hepatocyte MANF deletion activates iNOS/NF-κB in alcohol-induced liver injury. (a) Representative iNOS and p65 immunohistochemistry staining in liver tissues. Scale . (b) The relative mRNA level of iNOS was detected by using qPCR. (c) Nitric oxide (NO) level was detected with an assay kit. (d) Western blot analysis of hepatic expression levels of p65 and p-p65. (e) The quantitative data of p-p65 in (d). (f) The quantitative data of p65 in (d). The values were expressed as (–8). , , and . p-p65: phosphorylated p65.
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