Role of Mesencephalic Astrocyte-Derived Neurotrophic Factor in Alcohol-Induced Liver Injury

Figure 6

Hepatocyte MANF deletion activates oxidative stress in alcohol-induced liver injury. (a) Expression of hepatic cytochrome P450 2E1 (CYP2E1) in the liver tissues was detected by immunohistochemistry. Scale . (b) Expression of 4-HNE in the liver was detected by immunohistochemistry. Scale . (c) The protein levels of CYP2E1 and 4-HNE were detected by the western blot method. GAPDH was used as a loading control. (d) The quantitative data in (c). (e–g) The levels of MDA (e) and GSH (f) as well as the activity of SOD (g) in the liver tissues were detected with assay kits. The values were expressed as (–8). , , and .

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