Sesamol Protects Testis from Ischemia-Reperfusion Injury through Scavenging Reactive Oxygen Species and Upregulating CREMτ Expression

Figure 2

Analysis of CREMτ protein expression in ipsilateral (hatched histograms) and contralateral (open histograms) testes in sham-operated control, testicular ischemia-reperfusion (I-R), and sesamol-treated groups. (a) Representative Western blots of CREMτ protein expression in rat testes, where β-actin is used as loading control. Lanes 1L and 1R: left (i.e., ipsilateral) and right (i.e., contralateral) testes in sham-operated control group; Lanes 2L and 2R: ipsilateral and contralateral testes in testicular I-R group; Lanes 3L and 3R: ipsilateral and contralateral testes in sesamol-treated group. (b) The intensity ratio of CREMτ band to β-actin band from the same sample is considered as a relative expression level of CREMτ protein. Values () are presented as . means as compared with the control group. # means as compared with contralateral testes in the same group. § means as compared with ipsilateral testes in the I-R group.

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