Colchicine Alleviates Cholesterol Crystal-Induced Endothelial Cell Pyroptosis through Activating AMPK/SIRT1 Pathway

Figure 2

Colchicine alleviates NLRP3 inflammasome activation relevant in pyroptosis. HUVECs were treated for 24 h with different doses of colchicine (0–10 nM) in the presence of cholesterol crystal (0.5 mg/ml) or were left untreated (control). (a) Immunofluorescence was used for qualitative detection of NLRP3 (400x). (b) Western blotting was dedicated to examine the protein expression levels of NLRP3, ASC-1, procaspase-1, caspase-1, IL-18, and IL-1β. (c) Quantitative analysis of pyroptosis-associated protein expression. (d–i) Real-time PCR analysis of the mRNA levels of GSDMD and proinflammatory cytokines (IL-18, IL-1β, IL-6, IL-8, and MCP-1). Data was expressed as the of three separate experiments. , , , and vs. the control group. ^#, ^##, ^###, and ^#### vs. the cholesterol crystal group.

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