Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 5

Research Article

Targeting the Nrf2/ARE Signalling Pathway to Mitigate Isoproterenol-Induced Cardiac Hypertrophy: Plausible Role of Hesperetin in Redox Homeostasis

Figure 5

(a) Hesperetin suppressed intracellular ROS production. Representative images of DHE staining in H9c2 cells. Nuclei were counterstained with DAPI. Bar plots showing the fluorescence intensity (AU). (b) Nrf2 nuclear translocation in H9c2 cardiomyoblasts. Representative images of immunohistochemical staining for the levels of Nrf2. Histograms represent the quantification (nuclear) of fluorescence intensity. (c) Immunoblot analysis of Nrf2. Immunoblot analysis of Nrf2 levels in H9c2 cells. Actin was used as a loading control. Quantification of the protein levels normalized to actin is shown. Results are shown as the mean ± SEM of three separate experiments. Statistical significance (, , and ) was calculated by Student-Newman-Keuls and Tukey post hoc tests.

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