Research Article
The Effects and Mechanism of Quercetin Dietary Supplementation in Streptozotocin-Induced Hyperglycemic Arbor Acre Broilers
Table 3
Primers of genes related to the insulin signaling pathway used for mRNA expression level.
| | Name | Primer (5-3) | Sequence no. | Length (bps) |
| | β-Actin | Forward: GAGAAATTGTGCGTGACATCA | NM205518.1 | 152 bp | | Reverse: CCTGAACCTCTCATTGCCA |
| | IR | Forward: GACTCTCCAACGAACAGGTG | XM001233398.4 | 156 bp | | Reverse: TCAGCATCTCAATGACCTCAA |
| | IRS-1 | Forward: CAAGTTTGGACAGTGGAGCGT | XM003641084.3 | 106 bp | | Reverse: CAATGGTTTCCATGGCAATG |
| | PI3K | Forward: CGGATGTTGCCTTACGGTTGT | NM001004410.1 | 162 bp | | Reverse: GTTCTTGTCCTTGAGCCACTGAT |
| | PKB | Forward: CTGATGATGCCAAGGAGATT | NM205055.1 | 175 bp | | Reverse: TGGTCAGGAGGAGTGATTGT |
| | GSK-3β | Forward: AGCTGTTCCGGAGTTTAGCCTAT | XM004938179.2 | 151 bp | | Reverse: ACGTTAGGTTCTCCACGAACCA |
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