Effects of the Insulted Neuronal Cells-Derived Extracellular Vesicles on the Survival of Umbilical Cord-Derived Mesenchymal Stem Cells following Cerebral Ischemia/Reperfusion Injury
Hypoxia preconditioned enhances the survival of UC-MSCs upon OGD/R insult. UC-MSCs were cultured under hypoxia or normoxia preconditioning before non-OGD/R and OGD/R insult. (a–c) The expression of HIF-1α in normoxia and hypoxia preconditioned UC-MSCs group as evaluated western blotting and mRNA. (d) Expression and quantitative data of bax and bcl-2 were determined by western blotting. (e–f) Apoptosis of UC-MSCs as assessed by flow cytometry assay. (g) Apoptosis of UC-MSCs as determined by LDH leakage assay. (h) Cell viability of UC-MSCs as examined by MTT assay. (i) Levels of ROS as determined by dihydroethidium-DCFH-DA assay. (j) Levels of ATP as determined by firefly luciferase assay. (k) Levels of SOD as examined by WST-8 assay. (l) Levels of T-AOC as measured by ABTS assay. All data are presented as the ().;, hypoxia group compared with normoxia group.
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