LCZ696 Ameliorates Oxidative Stress and Pressure Overload-Induced Pathological Cardiac Remodeling by Regulating the Sirt3/MnSOD Pathway

Figure 5

LCZ696 treatment alleviated TAC-induced myocardial apoptosis. (a) Representative TUNEL staining images performed to assess myocardial apoptosis in mouse hearts in different groups. (b, c) Western blot analysis and quantitative analysis of Bcl-2 and Bax protein levels in different cardiac homogenates. (d) The protein expression of Sirt3 and MnSOD levels as detected by Western blot. (e) Histograms showing the quantitative expression of Sirt3 and MnSOD, . (f) Expression levels of Sirt3 and MnSOD in hearts from different groups. (g) Histogram of the quantitative analysis of the data in (f), after normalization to the expression of GAPDH, . (h) Western blot analysis of p-AMPK and AMPK protein levels in different cardiac homogenates. (i) Quantitative analysis of the ratio of p-AMPK/AMPK, . vs. the sham+vehicle group, vs. the sham+vehicle group, vs. the TAC+vehicle group.

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