PI3K/Akt and ERK1/2 Signalling Are Involved in Quercetin-Mediated Neuroprotection against Copper-Induced Injury
Effects of quercetin on caspase-3/7 activity and nuclear condensation. In moderately injured P19 neurons, a protective concentration of quercetin abolished copper-induced caspase-3/7 activation (a) and nuclear condensation (b), while in severely injured neurons, a prooxidative concentration of quercetin increased caspase activation without affecting chromatin condensation. Results of caspase-3/7 activity are expressed as from 4 independent experiments performed in duplicates. Changes in nuclear condensation were determined from 3 independent experiments. At least 500 nuclei per group were examined in each experiment, in randomly selected fields. a,c, and d vs. the copper-treated group; and vs. control (one-way ANOVA followed by post hoc Dunnett’s test).